4DMG
Thermus thermophilus m5C1942 methyltransferase RlmO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.797, 45.998, 129.364 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 27.371 - 1.700 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.989 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.065 | 0.043 | 0.424 |
| Number of reflections | 81685 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 98.7 | 94.1 | 99.8 |
| Redundancy | 3.2 | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 10% MPD, 50mM sodium citrate, 5mM AdoMet, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
