4DM7
Crystal structure of the CFTR inhibitory factor Cif with the E153D mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9782 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.353, 84.138, 89.166 |
| Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
| Resolution | 43.662 - 1.360 |
| R-factor | 0.2124 |
| Rwork | 0.212 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A OF PDB ENTRY 3KD2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.070 | 1.450 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 258302 | |
| <I/σ(I)> | 13.6 | 3.7 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 14% PEG 8000, 0.125M calcium chloride, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
