4DLW
H-Ras Set 2 Ca(OAc)2/DTT, on
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.395, 88.395, 133.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.282 - 1.720 |
| R-factor | 0.1768 |
| Rwork | 0.174 |
| R-free | 0.20240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.110 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.750 |
| High resolution limit [Å] | 1.720 | 4.670 | 1.720 |
| Rmerge | 0.054 | 0.022 | 0.666 |
| Number of reflections | 21602 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 100.0 | 99.3 | 100 |
| Redundancy | 10.4 | 9.8 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 3350, 200 mM Ca Acetate, pH 7.5, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
