4DLT
H-Ras Set 2 Ca(OAc)2, on
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 88.704, 88.704, 134.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.652 - 1.699 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.20440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.699 | 3.660 | 1.700 |
Rmerge | 0.065 | 0.033 | 0.865 |
Number of reflections | 22540 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 99.5 | 99.6 | 95 |
Redundancy | 7.9 | 8.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |