4DLR
H-Ras PEG 400/Ca(OAc)2, ordered off
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.415, 87.415, 133.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.924 - 1.319 |
| R-factor | 0.1704 |
| Rwork | 0.170 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.168 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.440 |
| High resolution limit [Å] | 1.319 | 3.850 | 1.420 |
| Rmerge | 0.059 | 0.035 | 0.335 |
| Number of reflections | 37269 | ||
| <I/σ(I)> | 17 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 10.9 | 10.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
