4DLN
Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9782 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.174, 83.953, 89.169 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 46.087 - 1.550 |
| R-factor | 0.1602 |
| Rwork | 0.159 |
| R-free | 0.17590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chain A of PDB ENTRY 3KD2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.087 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 173094 | |
| <I/σ(I)> | 16.9 | 4.9 |
| Completeness [%] | 98.0 | 96.7 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 16% PEG 8000, 0.125M calcium chloride, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
