4DKA
Structure of Editosome protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99990 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.653, 105.912, 40.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.970 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.910 | 2.030 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.088 | 0.390 |
| Number of reflections | 31254 | |
| <I/σ(I)> | 16.366 | 3.333 |
| Completeness [%] | 99.5 | 95.3 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.05 M ammonium sulfate, 0.05M sodium acetate trihydrate, 30% w/v PEG 2000 MME, 0.2 M sodium thiocyanate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
