4DK6
Structure of Editosome protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.026, 40.882, 61.281 |
| Unit cell angles | 90.00, 92.55, 90.00 |
Refinement procedure
| Resolution | 43.210 - 2.650 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7u |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.909 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
| Rmerge | 0.079 | 0.043 | 0.301 |
| Number of reflections | 12226 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 95.4 | 92.1 | 97.2 |
| Redundancy | 2.5 | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M magnesium chloride, 0.1M bis-tris, 25% w/v PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
