4DK3

Structure of Editosome protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	77
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 2
Unit cell lengths	95.790, 105.310, 94.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.030 - 2.760
R-factor	0.2264
Rwork	0.224
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.917
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.030	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.101	0.056	0.484
Number of reflections	12360
<I/σ(I)>	13.9
Completeness [%]	99.5	99.6	93.7
Redundancy	7	6.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.05 M ammonium sulfate, 0.05M sodium acetate trihydrate, 30% w/v PEG 2000 MME, 5% v/v jeffamine M600, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K