4DJT
Crystal structure of a nuclear GTP-binding protein from Encephalitozoon cuniculi bound to GDP-Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-13 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.570, 89.250, 54.980 |
Unit cell angles | 90.00, 108.06, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.1772 |
Rwork | 0.175 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qg4 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.395 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 | |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.038 | 0.024 | 0.308 |
Number of reflections | 34910 | 429 | 2375 |
<I/σ(I)> | 34.93 | 111.61 | 5.6 |
Completeness [%] | 96.3 | 98.4 | 89.1 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | EncuA.01002.a.A1 PS00532 at 44.6 mg/mL with 5 mM GDP against CSHT screen condition H2: 0.1 M HEPES, pH 7.5, 20% PEG10000, cryoprotectant: 20% ethylene glycol, crystal tracking ID 225457h2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |