4DJN
Crystal structure of a ribonucleotide reductase M2 B (RNRR2) from Homo sapiens at 2.20 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.053, 98.762, 133.668 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.834 - 2.200 |
R-factor | 0.1945 |
Rwork | 0.193 |
R-free | 0.21910 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.173 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.834 | 29.834 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.053 | 0.730 | |
Total number of observations | 4513 | 12292 | |
Number of reflections | 45830 | ||
<I/σ(I)> | 9.8 | 32.8 | 1.7 |
Completeness [%] | 97.5 | 94.2 | 95.8 |
Redundancy | 6.3 | 7.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 2.0M ammonium sulfate, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |