4DJN

Crystal structure of a ribonucleotide reductase M2 B (RNRR2) from Homo sapiens at 2.20 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-08
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	69.053, 98.762, 133.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.834 - 2.200
R-factor	0.1945
Rwork	0.193
R-free	0.21910
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.173
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.834	29.834	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge		0.053	0.730
Total number of observations		4513	12292
Number of reflections	45830
<I/σ(I)>	9.8	32.8	1.7
Completeness [%]	97.5	94.2	95.8
Redundancy	6.3	7.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	2.0M ammonium sulfate, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K