4DJK
Structure of glutamate-GABA antiporter GadC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.962, 106.408, 185.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.140 - 3.097 |
| R-factor | 0.2849 |
| Rwork | 0.282 |
| R-free | 0.32460 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.097 | 3.097 |
| Rmerge | 0.054 | 0.428 |
| Number of reflections | 21370 | |
| <I/σ(I)> | 26.8 | 3.1 |
| Completeness [%] | 79.5 | 19.9 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 25% PEG400, 100mM Tris-HCl, 200mM NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
