4DJC

1.35 A crystal structure of the NaV1.5 DIII-IV-Ca/CaM complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-08
Detector	RAYONIX MX300HE
Wavelength(s)	0.9749
Spacegroup name	P 21 21 21
格子定数 [Å]	28.380, 72.720, 97.010
格子定数 [度]	90.00, 90.00, 90.00

精密化法

分解能	29.550 - 1.350
R因子	0.15391
Rwork	0.153
R-free	0.17626
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.620
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.380
分解能 [Å] (高)	1.350	1.350
独立反射数	44657
完全性 [%]	99.0	92.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1M MES, 60% Isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K