4DJ8
Structure of the hemagglutinin complexed with 6SLN from a highly pathogenic H7N7 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 203.774, 117.603, 120.456 |
Unit cell angles | 90.00, 124.15, 90.00 |
Refinement procedure
Resolution | 48.230 - 2.800 |
R-factor | 0.19251 |
Rwork | 0.190 |
R-free | 0.23494 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.863 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 54989 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 298 | 20 % PEG 8000 and 1 M lithium chloride, microbatch under oil, temperature 298K, EVAPORATION |