4DJ3
Unwinding the Differences of the Mammalian PERIOD Clock Proteins from Crystal Structure to Cellular Function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.650, 78.640, 67.170 |
| Unit cell angles | 90.00, 97.63, 90.00 |
Refinement procedure
| Resolution | 48.734 - 2.500 |
| R-factor | 0.21649 |
| Rwork | 0.213 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.806 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 22419 | |
| Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 100 mM MES, 18% PEG 6000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
