4DIU

Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR94

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-01-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	64.447, 95.595, 50.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.660 - 2.000
R-factor	0.214
Rwork	0.214
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	17960
Completeness [%]	92.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch under oil	6	277	2.03 M Lithium sulfate monohydrate .1M MES pH 6, Microbatch under oil, temperature 4 K, temperature 277K