4DIM
Crystal structure of phosphoribosylglycinamide synthetase from Anaerococcus prevotii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.270, 84.467, 49.642 |
| Unit cell angles | 90.00, 100.54, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.610 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.22790 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.089 | 0.599 |
| Number of reflections | 15567 | |
| <I/σ(I)> | 24.4 | 2.35 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1 M Bis-Tris/HCl, 20% PEG MME 5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
