4DI3
Crystal structure of a 2:1 complex of Treponema pallidum TatP(T) (Tp0957) bound to TatT (Tp0956)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979347 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 148.934, 148.934, 212.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.650 - 3.050 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u64 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.233 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 6.570 | 3.050 |
| Rmerge | 0.055 | 0.020 | 0.970 |
| Number of reflections | 45743 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.0 | 98.3 | 98.8 |
| Redundancy | 5.1 | 4.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 293 | 0.1 M CHES, 30% PEG400, pH 9.5, VAPOR DIFFUSION, temperature 293K |
