4DHW

Crystal structure of Peptidyl-tRNA hydrolase from Pseudomonas aeruginosa with Adipic acid at 2.4 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2012-01-03
Detector	MARRESEARCH
Wavelength(s)	1.514
Spacegroup name	P 61
Unit cell lengths	64.594, 64.594, 156.245
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.940 - 2.430
Rwork	0.195
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p2j
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.940	2.520
High resolution limit [Å]	2.430	2.430
Number of reflections	13898
<I/σ(I)>	10.5	2
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M HEPES pH 8.5, PEG 4000, 5% Isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K