4DHG
Crystal structure of enolase TBIS_1083(TARGET EFI-502310) from Thermobispora bispora dsm 43833, an open loop conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.028, 84.823, 94.119 |
| Unit cell angles | 89.26, 84.07, 72.72 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.16214 |
| Rwork | 0.161 |
| R-free | 0.20031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vc5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 189335 | |
| <I/σ(I)> | 9.7 | 1.3 |
| Completeness [%] | 96.9 | 98.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M SODIUM IODIDE, PH 7.5, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
