4DHE
Crystal structure of a probable GTP-binding protein engB from Burkholderia thailandensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97740 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.940, 76.960, 134.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.781 - 2.200 |
R-factor | 0.2368 |
Rwork | 0.235 |
R-free | 0.27380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pui |
RMSD bond length | 0.012 |
RMSD bond angle | 1.443 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 | |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.093 | 0.024 | 0.497 |
Number of reflections | 26282 | 294 | 1906 |
<I/σ(I)> | 13.31 | 42.94 | 2.99 |
Completeness [%] | 99.0 | 83.5 | 98.8 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | ButhA.00252.a.A1.PW33397 at 34 mg/mL against JCSG+ H8, 0.2 M NaCl, 0.1 M BisTris, 25% PEG 3350 with 15% EG as cryo-protectant, Crystal tracking ID 225992h8, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |