4DH2
Crystal structure of Coh-OlpC(Cthe_0452)-Doc435(Cthe_0435) complex: A novel type I Cohesin-Dockerin complex from Clostridium thermocellum ATTC 27405
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-24 |
Detector | ADSC QUANTUM 4r |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 90.760, 90.760, 135.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.300 - 1.750 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ccl |
RMSD bond length | 0.014 |
RMSD bond angle | 1.296 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | DM (6.0) |
Refinement software | PHENIX (1.7.2_865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.600 | 1.840 |
High resolution limit [Å] | 1.745 | 1.740 |
Rmerge | 0.887 | |
Number of reflections | 66027 | |
<I/σ(I)> | 18.7 | 2.4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 14.9 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 293 | 2 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |