4DGB

TRIMCyp cyclophilin domain from Macaca mulatta: HIV-2 CA cyclophilin-binding loop complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-07-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	36.170, 55.720, 70.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.340 - 1.702
R-factor	0.1791
Rwork	0.177
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wlw
RMSD bond length	0.011
RMSD bond angle	1.220
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.080	32.310	1.790
High resolution limit [Å]	1.702	5.380	1.702
Rmerge		0.024	0.108
Total number of observations		2268	9350
Number of reflections	15839
<I/σ(I)>	14.9	25.8	6.9
Completeness [%]	95.0	98.2	91.5
Redundancy	2.3	2.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	290	2.35 M ammonium phosphate dibasic, 0.1 M Tris-HCl, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 290K