4DG7
Low resolution structure of Drosophila Translin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.050, 131.200, 96.400 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 48.146 - 4.195 |
| R-factor | 0.2402 |
| Rwork | 0.237 |
| R-free | 0.29220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.275 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 95.000 |
| High resolution limit [Å] | 4.195 |
| Rmerge | 0.132 |
| Number of reflections | 16416 |
| <I/σ(I)> | 5.6 |
| Completeness [%] | 99.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 4000, 100mM bicine (pH 8.5), 200mM sodium formate, 5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
