4DF3
Crystal Structure of Aeropyrum pernix fibrillarin in complex with natively bound S-adenosyl-L-methionine at 1.7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-01 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.639, 52.087, 63.030 |
| Unit cell angles | 66.28, 84.55, 77.61 |
Refinement procedure
| Resolution | 46.780 - 1.730 |
| R-factor | 0.18954 |
| Rwork | 0.187 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.907 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.780 |
| High resolution limit [Å] | 1.730 |
| Rmerge | 0.066 |
| Number of reflections | 47059 |
| <I/σ(I)> | 0.189 |
| Completeness [%] | 90.6 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296.15 | 20mM Tris.HCL pH8, 150mM NaCl, 200mM Imidazole, 20mM Tris.HCl pH7, 5% Isopropanol, 10% PEG 4000, pH 6.5, Vapor DIffusion, Sitting Drop, temperature 296.15K |
