4DEJ
Crystal structure of glutathione transferase-like protein IL0419 (Target EFI-501089) from Idiomarina loihiensis L2TR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.356, 156.988, 244.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.20951 |
| Rwork | 0.207 |
| R-free | 0.27533 |
| Structure solution method | MR |
| Starting model (for MR) | 1yy7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.248 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 87401 | |
| <I/σ(I)> | 6.9 | 0.7 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 6.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2M AMMONIUM SULFATE, 0.1M HEPES-NA, 30% PEG8000', pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
