4DE8
LytR-Cps2a-Psr family protein with bound octaprenyl monophosphate lipid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.187, 73.187, 163.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.720 - 1.950 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.782 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.364 | |
| Number of reflections | 33316 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.2M diammonium citrate 15% PEG 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
