4DE6

Horse spleen apo-ferritin complex with arachidonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-06-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	180.830, 180.830, 180.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.320 - 2.180
R-factor	0.2023
Rwork	0.200
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xz1
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.320	2.250
High resolution limit [Å]	2.180	2.180
Rmerge	0.090	0.458
Number of reflections	12950
<I/σ(I)>	19.6	2.8
Completeness [%]	1.0	99.3
Redundancy	20.6	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	(NH4)2SO4 and CDSO4, ,0.2 mM arachidonate was mixed in the reservoir, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K