4DE5
Pantothenate synthetase in complex with fragment 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.480, 71.040, 82.020 |
Unit cell angles | 90.00, 99.56, 90.00 |
Refinement procedure
Resolution | 80.850 - 2.250 |
R-factor | 0.15193 |
Rwork | 0.148 |
R-free | 0.22304 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.769 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.850 |
High resolution limit [Å] | 2.250 |
Rmerge | 0.048 |
Number of reflections | 26165 |
<I/σ(I)> | 22.8 |
Completeness [%] | 100.0 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 12-14% PEG 3000, 150 mM Li2SO4, 100 mM imidazole, 2% Ethanol, 10% Glycerol,20 mM MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |