4DDQ
Structural plasticity of the Bacillus subtilis GyrA homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 152.803, 165.106, 180.582 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.020 - 3.300 |
R-factor | 0.177 |
Rwork | 0.174 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ab4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.296 |
Data reduction software | XDS ((VERSION December 6) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_934)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.020 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.172 | 0.858 |
Number of reflections | 69239 | |
<I/σ(I)> | 7.37 | 1.06 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.3 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 295 | 0.1M Tris/HCl pH 9.0, 25% P400, 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 295K |