4DCU
Crystal Structure of B. subtilis EngA in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.060, 65.290, 111.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.000 |
| R-factor | 0.21161 |
| Rwork | 0.206 |
| R-free | 0.25841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hjg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.213 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.469 |
| Number of reflections | 31287 | |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 98.0 | 89.8 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12% w/v PEG 3350, 100 mM Mes, 100 mM NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
