4DCT
Crystal Structure of B. subtilis EngA in complex with half-occupacy GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.760, 65.260, 112.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.970 - 2.300 |
R-factor | 0.23138 |
Rwork | 0.224 |
R-free | 0.29518 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hjg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.261 |
Data reduction software | XDS (package) |
Data scaling software | XDS (package) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.103 | 0.486 |
Number of reflections | 20375 | |
<I/σ(I)> | 11.9 | 4.5 |
Completeness [%] | 96.2 | 92.2 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12% PEG 3350 w/v, 100 mM Mes, 100 mM NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |