4DCT
Crystal Structure of B. subtilis EngA in complex with half-occupacy GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.760, 65.260, 112.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.970 - 2.300 |
| R-factor | 0.23138 |
| Rwork | 0.224 |
| R-free | 0.29518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hjg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.261 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.103 | 0.486 |
| Number of reflections | 20375 | |
| <I/σ(I)> | 11.9 | 4.5 |
| Completeness [%] | 96.2 | 92.2 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12% PEG 3350 w/v, 100 mM Mes, 100 mM NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
