4DCI
Crystal structure of unknown funciton protein from Synechococcus sp. WH 8102
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.272, 164.522, 105.421 |
| Unit cell angles | 90.00, 131.80, 90.00 |
Refinement procedure
| Resolution | 91.750 - 2.820 |
| R-factor | 0.18063 |
| Rwork | 0.177 |
| R-free | 0.25154 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.750 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.109 | 0.808 |
| Number of reflections | 44991 | |
| <I/σ(I)> | 20.3 | 1.69 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 289 | 1.8M Magnesium sulfate, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
