Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.938, 88.465, 62.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.381 - 1.800 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.22460 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.147 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 33721 | |
<I/σ(I)> | 36.09 | 2.23 |
Completeness [%] | 99.4 | 99 |
Redundancy | 5.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M MG CHLORIDE, 0.1 M BIS-TRIS PH 6.5, 25% PEG3350, 2.5 MM ATP, VAPOR DIFFUSION, SITTING DROP, temperature 298K |