4DC9
Hexameric ring of Methanococcus voltae RadA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-15 |
| Detector | Brukers Proteum |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.351, 118.577, 141.731 |
| Unit cell angles | 90.00, 138.05, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.20916 |
| Rwork | 0.206 |
| R-free | 0.26584 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.293 |
| Number of reflections | 57374 | |
| <I/σ(I)> | 8.5 | 2.6 |
| Completeness [%] | 90.5 | 90.1 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 294 | 33% PEG 400, 1.0 M NaNO3, 0.05 M MES-NaOH Buffer at pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
