4DC5

Crystal Structure of Thaumatin Unexposed to Excessive SONICC Imaging Laser Dose.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	57.910, 57.910, 150.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.890 - 1.480
R-factor	0.175
Rwork	0.175
R-free	0.19100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2vi3
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.520
High resolution limit [Å]	1.480	1.480
Number of reflections	43152
Completeness [%]	98.9	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	sodium potassium tartrate, 5% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K