4DBR
Myosin VI D179Y (MD) pre-powerstroke state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.940, 93.530, 102.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.361 - 1.950 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.257 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.361 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 69466 | |
| Completeness [%] | 99.6 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 50 mM TrisHCl pH 8, 5.5% PEG8K, 50 mM KSCN, 5% glycerol, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
