4DBF
Crystal structures of Cg1458
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.120, 124.120, 73.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.19645 |
| Rwork | 0.194 |
| R-free | 0.24365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfu |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.010 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 87.770 | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 2.000 | 1.900 |
| Number of reflections | 45001 | ||
| Completeness [%] | 98.4 | 98.4 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.2M MgCl2, 0.1M Bis-Tris pH 6.0, 25% w/v Polyethylene Glycol 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 295K |
