4DBD

Crystal structure of orotidine 5'-monophosphate decarboxylase from Sulfolobus solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	40.367, 64.819, 73.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.618 - 1.699
R-factor	0.2097
Rwork	0.208
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ve9
RMSD bond length	0.006
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.618
High resolution limit [Å]	1.699
Number of reflections	21586
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	1.0M lithium sulfate, 0.1M sodium citrate, 0.5M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K