4DBA
Designed Armadillo repeat protein (YIIM3AII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.151, 60.603, 61.862 |
| Unit cell angles | 74.82, 89.55, 75.53 |
Refinement procedure
| Resolution | 25.288 - 2.400 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.30140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.203 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.560 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.057 |
| Number of reflections | 26658 |
| <I/σ(I)> | 2.1 |
| Completeness [%] | 92.8 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.05 M succinic acid, pH 4.0, 20% PEG 4000, 0.2 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
