4DB8
Designed Armadillo-repeat Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.002, 113.643, 85.592 |
| Unit cell angles | 90.00, 106.83, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.23563 |
| Rwork | 0.232 |
| R-free | 0.29885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.999 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.110 |
| Number of reflections | 33307 |
| <I/σ(I)> | 2.3 |
| Completeness [%] | 86.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M magnesium chloride, 0.1 M HEPES,pH 7.5, 30% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
