4DB4

Mss116p DEAD-box helicase domain 2 bound to a chimaeric RNA-DNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	77
Detector technology	CCD
Collection date	2011-05-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	43.696, 70.112, 214.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.818 - 3.599
R-factor	0.2482
Rwork	0.245
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.005
RMSD bond angle	0.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.599	3.599
Rmerge	0.129	0.657
Number of reflections	8213
<I/σ(I)>	13.2	2.7
Completeness [%]	99.6	98
Redundancy	7.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	6% tacsimate, pH 5.0, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K