4DB2

Mss116p DEAD-box helicase domain 2 bound to an RNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	77
Detector technology	IMAGE PLATE
Collection date	2011-08-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	160.534, 88.422, 121.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.624 - 3.157
R-factor	0.2266
Rwork	0.224
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PSB ENTRY 3I5X
RMSD bond length	0.002
RMSD bond angle	0.634
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	121.238	3.270
High resolution limit [Å]	3.150	3.150
Rmerge	0.103	0.467
Number of reflections	30146
<I/σ(I)>	11.7	2.3
Completeness [%]	99.4	94.8
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	8% tacsimate, pH 6.0, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K