4DAV
The structure of Pyrococcus Furiosus SfsA in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97957200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.700, 42.690, 88.900 |
| Unit cell angles | 90.00, 104.92, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.200 |
| R-factor | 0.24248 |
| Rwork | 0.240 |
| R-free | 0.29788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4da2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 29894 | |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 3.8mM CaCl2 in 6% (w/v) PEG 6000, 0.2 M citric acid, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
