4D9J
Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 122.700, 187.410, 283.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.893 - 3.920 |
| R-factor | 0.2828 |
| Rwork | 0.282 |
| R-free | 0.29360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vdx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.893 | 19.893 | 4.020 |
| High resolution limit [Å] | 3.920 | 17.530 | 3.920 |
| Rmerge | 0.066 | 0.030 | 0.317 |
| Number of reflections | 58471 | 227 | 3837 |
| <I/σ(I)> | 21.13 | 42.5 | 6.29 |
| Completeness [%] | 98.2 | 29.3 | 88.5 |
| Redundancy | 7.097 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.3 | 298 | 1.2M NaH2PO4, 0.8M K2HPO4, 0.1M CAPS, pH 10.3, vapor diffusion, hanging drop, temperature 298K |
