4D8J
Structure of E. coli MatP-mats complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.870, 180.100, 184.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.580 - 3.550 |
| R-factor | 0.299 |
| Rwork | 0.299 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vea |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 180.100 | 3.770 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.084 | 0.236 |
| Number of reflections | 36461 | |
| <I/σ(I)> | 7 | 2.2 |
| Completeness [%] | 88.0 | 80.6 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 5% PEG 1000, 0.1 M acetate, pH 5.0, 40% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
