4D8G
Chlamydia trachomatis NrdB with a Mn/Fe cofactor (procedure 2 - low Mn)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.85, 1.72, 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.387, 98.264, 100.390 |
Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
Resolution | 29.740 - 1.750 |
R-factor | 0.1949 |
Rwork | 0.194 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.238 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
Number of reflections | 143082 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 3000, sodium acetate, pH 7.4, vapor diffusion, hanging drop, temperature 298K |