4D87
Crystal Structure of Tyrosinase from Bacillus megaterium in complex with SDS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Detector technology | CCD |
| Collection date | 2011-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.840, 79.140, 85.930 |
| Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
| Resolution | 83.500 - 3.500 |
| R-factor | 0.26248 |
| Rwork | 0.259 |
| R-free | 0.29287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 83.530 | 57.450 | 3.690 |
| High resolution limit [Å] | 3.500 | 11.070 | 3.500 |
| Rmerge | 0.097 | 0.058 | 0.162 |
| Number of reflections | 6631 | ||
| <I/σ(I)> | 9.7 | 7.8 | |
| Completeness [%] | 83.0 | 93.3 | 73.4 |
| Redundancy | 2.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
