4D7Y
Crystal structure of mouse C1QL1 globular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 |
Unit cell lengths | 68.080, 68.080, 65.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.040 - 1.440 |
R-factor | 0.1415 |
Rwork | 0.140 |
R-free | 0.16930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c28 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.096 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.940 | 1.480 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmerge | 0.070 | 0.880 |
Number of reflections | 20630 | |
<I/σ(I)> | 16.9 | 2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.6 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 0.02M NICKEL(II) CHLORIDE HEXAHYDRATE, 0.02M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.02M CADMIUM CHLORIDE HYDRATE, 0.1M SODIUM ACETATE TRIHYDRATE PH 4.5, 24% W/V POLYETHYLENE GLYCOL, MONOMETHYL ETHER 2,000 |