4D7V
The structure of the catalytic domain of NcLPMO9C from the filamentous fungus Neurospora crassa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-15 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.308, 67.528, 65.592 |
Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
Resolution | 64.406 - 1.900 |
R-factor | 0.215 |
Rwork | 0.212 |
R-free | 0.26720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SAME PROTEIN WITH ONE METAL INSTEAD OF 3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.447 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.410 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.310 | 1.270 |
Number of reflections | 29831 | |
<I/σ(I)> | 6.8 | 2 |
Completeness [%] | 99.3 | 98.4 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.2M ZINC ACETATE, 17.5% PEG3350, pH 8 |